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CD45 Mouse, Brilliant Violet 421, Clone: D058-1283, BD Optibuild™

Catalog No. BDB740084 Shop All BD Biosciences Products
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BDB740084 50 μg
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Catalog No. BDB740084 Supplier BD Biosciences Supplier No. 740084
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Description

Mouse Monoclonal Antibody

D058-1283 is a CD45 monoclonal antibody specific for non-human primate leucocytes. It was developed using Rhesus peripheral whole blood as the immunogen. It does not cross-react with human leucocytes. This antibody reacts with baboon, Rhesus and Cynomolgus Macaque leucocytes in a similar pattern to CD45 binding to leukocyte common antigen (LCA) on human cells. Immunophenotypic analysis shows that D058-1283 binds to lymphocytes, monocytes and granulocytes of non-human primate blood samples. This antibody is able to block the binding of monoclonal antibody TÜ116; a reported anti-human CD45 antibody that cross-reacts with nonhuman primate leucocytes. In Western blot analysis, the D058-1283 antibody identifies a 180-200 kDa band.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD45
Applications Flow Cytometry
Classification Monoclonal
Clone D058-1283
Concentration 0.2mg/mL
Conjugate Brilliant Violet 421
Description Pan Leukocyte, NHP-specific; PTPRC; LCA; L-CA; Leukocyte Common Ag
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Mouse
Immunogen Rhesus peripheral whole blood
Purification Method Affinity Purified
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Baboon, Cynomolgus, Rhesus Monkey
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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