MilliporeSigma™ Mouse anti-NQO2, Clone: 4A4.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABC1038

Catalog No. MABC1038

Description

Specifically detects NQO2 clone: 4A4.1 in Human samples, and it is validated for Immunohistochemistry, Western Blotting

Ribosyldihydronicotinamide dehydrogenase [quinone] (EC 1.10.5.1; UniProt P16083; also known as NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2) is encoded by the NQO2 (also known as NMOR2) gene (Gene ID 4835) in human. NQO1 (EC 1.6.5.2) and NQO2 (EC 1.10.5.1; formerly EC 1.10.99.2) constitute the quinone oxidoreductase family in human. NQO1 is involved in vitamin K metabolism and in reducing the cellular quinone concentration to prevent the build-up of cellular reactive oxygen species (ROS). Both enzymes contain a tightly bound FAD as the oxidant cofactor, but differ in their reductant cofactors utilization. While NQO1 can efficiently utilize NADH or NADPH, NQO2 exhibits only low activity in the presence of NADH or NADPH. Instead, non-physiological compounds, such as dihydronicotinamide riboside (NRH), N-methyldihydronicotinamide, and dihydrobenzylnicotamide, are reported to act as good reductant cofactors in NQD2-catalyzed reactions in vitro. NQO1 & NQO2 catalyzed the reduction of a wide variety of substrates, including quinones, nitro-compounds, and iron (III) ions. NQO1 is inhibited by the anticoagulant dicoumarol, while NQO2 is only weakly inhibited by dicoumarol and more strongly inhibited by resveratrol.

Specifications

• Antigen: NQO2
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: NQO2; NMOR2
• Immunogen: GST-tagged recombinant human NQO2 N-terminal fragment.
• Quantity: 100 μg
• Research Discipline: Apoptosis, Cancer
• Gene ID (Entrez): NP_000895
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG2b κ

• Applications: Immunohistochemistry, Western Blot
• Clone: 4A4.1
• Gene Accession No.: P16083
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

For Research Use Only