MilliporeSigma™ Mouse anti-Oct-1 (Ascites Free), Clone: YL15,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MAB5434C

Catalog No. MAB5434C

Description

Specifically detects Oct-1 (Ascites Free) clone: YL15 in Human, Mouse samples, and it is validated for ChIP, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blotting

POU domain, class 2, transcription factor 1 (UniProt P14859; also known as NF-A1, Oct-1, Octamer-binding protein 1, Octamer-binding transcription factor 1, OTF-1) is encoded by the POU2F1 (also known as OCT1, OTF1) gene (Gene ID 5451) in human. Oct-1 belongs to the family of octamer transcription factors characterized by their binding to the ATTTGCAT octamer sequence via their POU domain (a.a. 280-354 of human Oct-1). Oct1 is widely expressed in adult tissues, however, Oct-1 knockdown has little impact on cell growth and viability in culture. Oct-1 controls multiple stem cell phenotypes in normal and tumor cells. Oct-1 promotes resistance to genotoxic and oxidative stresses in stem cells. Loss of Oct-1 antagonizes oncogenic transformation in vitro and tumorigenicity in vivo.

Specifications

• Antigen: Oct-1 (Ascites Free)
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: POU2F1; OCT1; OTF1
• Immunogen: Recombinant full-length human Oct-1.
• Quantity: 100 μg
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_001185712
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG1 κ

• Applications: ChIP Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Clone: YL15
• Gene Accession No.: P14859
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Form: Purified

For Research Use Only