MilliporeSigma™ Mouse anti-PAFAH 1B3, Clone: 3E4.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1188

Catalog No. MABS1188

Description

Specifically detects PAFAH 1B3 clone: 3E4.1 in Human, Mouse samples, and it is validated for Immunocytochemistry, Western Blotting

Platelet-activating factor acetylhydrolase IB subunit gamma (EC 3.1.1.47; UniProt Q15102; also known as PAF acetylhydrolase 29kDa subunit, PAF-AH 29kDa subunit, PAF-AH1b subunit alpha 1, PAF-AH subunit gamma, PAFAH subunit gamma) is encoded by the PAFAH1B3 (also known as PAFAHG) gene (Gene ID 5050) in human. Platelet-activating factor (PAF) acetylhydrolase (PAF-AH) negatively regulates PAF potency by catalyzing the removal of the acetyl group at the sn-2 position of the lipid messenger. There are two types of intracellular PAF-AH and a plasma type PAF-AH. Type I intracellular PAF-AH is a heterotrimeric G-protein-like complex composed of alpha1 (originally called gamma), alph2 (originally called alpha), and beta subunits. Both alpha1 and alpha2 contains a catalytic site. The alpha2/2 homodimer hydrolyzes PAF (1-O-alkyl-2-acetyl-sn-glycero-3-PC) and 1-O-alkyl-2-acetyl-sn-glycero-3-PE more efficiently, while the alpha1/1 homodimer and alpha1/2 heterodimer are more efficient toward 1-O-alkyl-2-acetyl-sn-glycero-3-PA than PAF. The alpha 1 subunit is reported to be up-regulated in primary human breast tumors and correlated with poor prognosis. Inactivation of alpha 1 subunit enhances cellular level of tumor-suppressing signaling lipids.

Specifications

• Antigen: PAFAH 1B3
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: PAFAH1B3; PAFAHG
• Immunogen: GST-tagged recombinant internal fragment from the C-terminal half of human PAFAH1B3.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_001139411
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG2b κ

• Applications: Immunocytochemistry, Western Blot
• Clone: 3E4.1
• Gene Accession No.: Q15102
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Form: Purified

For Research Use Only