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Mouse anti-pan Actin, Clone: HHF35, Azide Free, Novus Biologicals™
SDP

Catalog No. p-5323042 Shop All R&D Systems Products
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0.2 mg
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NBP23463402 0.2 mg
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Catalog No. NBP23463402 Supplier Novus Biologicals Supplier No. NBP2346340.2 MG

Mouse Monoclonal Antibody

pan Actin Monoclonal antibody specifically detects pan Actin in Human, Mouse, Rat, Canine, Chicken, Feline, Rabbit samples. It is validated for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

Antigen pan Actin
Applications Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone HHF35
Concentration 1.0 mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, CyTOF-ready
Formulation 10mM PBS - BSA free with No Preservative
Gene Accession No. P62736
Gene Alias ACTA, actin, alpha 1, skeletal muscle, alpha skeletal muscle, alpha skeletal muscle actin, alpha-actin-1, ASMA, CFTD, CFTDM, MPFD, NEM1, NEM2, NEM3
Gene Symbols ACTA1
Host Species Mouse
Immunogen SDS extract of human myocardium.
Purification Method Protein A purified
Quantity 0.2 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 58
Test Specificity This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Reconstitution Protein A purified
Target Species Human, Mouse, Rat, Canine, Chicken, Feline, Rabbit
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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