Mouse anti-pan Cytokeratin, Clone: AE1 + AE3, Azide Free, Novus Biologicals™

Supplier:  Novus Biologicals NBP2332000.2MG

Catalog No. NBP23320002

Specifications

• Antigen: pan Cytokeratin
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 0.5 - 1 ug/ml, Flow Cytometry 0.1 - 1 ug/million cells, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunohistochemistry-Frozen 0.25 - 0.5 ug/ml, Immunofluorescence 1 - 2 ug/ml, CyTOF-ready
• Gene Symbols: KRT1
• Immunogen: Total keratin isolated from human epidermal callus was used as immunogen to generate the pan cytokeratin antibodies AE1 + AE3 (Woodcock-Mitchell, 1982).
• Quantity: 0.2 mg
• Research Discipline: Cytoskeleton Markers, Protein Kinase, Signal Transduction
• Gene ID (Entrez): 3848
• Target Species: Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence
• Clone: AE1 + AE3
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

For Research Use Only