MilliporeSigma™ Mouse anti-PD-L1, Clone: 29E.2A3.C6,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABC980

Catalog No. MABC980

Description

Specifically detects PD-L1 clone: 29E.2A3.C6 in Chimpanzee, Human, Rat samples, and it is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Neutralization

Programmed cell death 1 ligand 1 (UniProt Q9NZQ7; also known as B7-H1, B7 homolog 1, CD274, PD-L1, PDCD1 ligand 1, Programmed death ligand 1) is encoded by the CD274 (also known as B7H1, PDCD1L1, PDCD1LG1, PDL1) gene (Gene ID 29126) in human. PD-1 and PD-1 ligands 1&2 (PD-L1 and PD-L2) are B7:CD28 family members that regulate T cell activation and peripheral tolerance. When engaged together with the TCR, the interaction of PD-1 with its ligands delivers an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and nonhematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Under some conditions, blockade of this pathway is able to restore many T cell functions. PD-L1 is initially produced with signal peptide (a.a. 1-18) sequence, the removal of which yields the mature protien with a large extracellular (a.a. 19-238) region that contains an Ig-like V-type domain (a.a. 19-127) and an Ig-like C2-type domain (a.a. 133-225), followed by a transmembrane domain (a.a. 239-259) and a cytoplasmic tail (a.a. 260-290).

Specifications

• Antigen: PD-L1
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG2bκ antibody in PBS without preservatives.
• Gene Symbols: CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1
• Immunogen: Recombinant full-length human PD-L1.
• Quantity: 100 μg
• Research Discipline: Apoptosis, Cancer
• Gene ID (Entrez): NP_054862.1
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
• Isotype: IgG2b κ

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Neutralization
• Clone: 29E.2A3.C6
• Gene Accession No.: Q9NZQ7
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Chimpanzee, Human, Rat
• Form: Purified

For Research Use Only