MilliporeSigma™ Mouse anti-PD-L2, Clone: 24F.10C12,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABC969

Catalog No. MABC969

Description

Specifically detects PD-L2 clone: 24F.10C12 in Human samples, and it is validated for Flow Cytometry, Immunohistochemistry, Neutralization, Western Blotting

Programmed cell death 1 ligand 2 (UniProt Q9BQ51; also known as B7-DC, B7 dendritic cell molecule, B7-H2, Butyrophilin B7-DC, CD273, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2) is encoded by the PDCD1LG2 (also known as B7DC, CD273, PDCD1L2, PDL2) gene (Gene ID 80380) in human. PD-1 and PD-1 ligands 1&2 (PD-L1 and PD-L2) are B7:CD28 family members that regulate T cell activation and peripheral tolerance. When engaged together with the TCR, the interaction of PD-1 with its ligands delivers an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and nonhematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Under some conditions, blockade of this pathway is able to restore many T cell functions. PD-L2 is initially produced with signal peptide (a.a. 1-19) sequence, the removal of which yields the mature protien with a large extracellular (a.a. 20-220) region that contains an Ig-like V-type domain (a.a. 21-118) and an Ig-like C2-type domain (a.a. 122-203), followed by a transmembrane domain (a.a. 221-241) and a cytoplasmic tail (a.a. 242-273).

Specifications

• Antigen: PD-L2
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG2aκ antibody in buffer containing PBS without preservatives.
• Gene Symbols: PDCD1LG2; B7DC; CD273; PDCD1L2; PDL2
• Immunogen: Recombinant human PD-L2.
• Quantity: 100 μg
• Research Discipline: Apoptosis, Cancer
• Gene ID (Entrez): NP_079515.2
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
• Isotype: IgG2a κ

• Applications: Flow Cytometry, Immunohistochemistry, Neutralization, Western Blot
• Clone: 24F.10C12
• Gene Accession No.: Q9BQ51
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

For Research Use Only