MilliporeSigma™ Mouse anti-PL Scramblase 1, Clone: 4D2,

Catalog No.	Quantity
MABS483MI	100 μg

Catalog No. MABS483MI Supplier MilliporeSigma™ Supplier No. MABS483

Description

Mouse Monoclonal Antibody

Specifically detects PL Scramblase 1 clone: 4D2 in Human samples, and it is validated for Immunocytochemistry, Immunoprecipitation, Western Blotting

Phospholipid scramblase 1 (UniProt O15162; also known as Ca²⁺-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL scramblase 1) is encoded by the PLSCR1 (also known as MMTRA1B) gene (Gene ID 5359) in human. Plasma membrane phospholipids are distributed asymmetrically between the inner and outer leaflets. Such asymmetrical distribution collapses in response to blood coagulation and apoptosis, resulting in phospholipid 'scrambling' between the two leaflets of the plasma membrane. Flippases, floppases, and scramblases are three types enzymes known to mediate transbilayer lipid motion. Flippases and floppases function via an ATP-dependent mechanism, while scramblases mediate transbilayer movement in a non-selective and energy-independent manner. Originally identified in 1996 as a 37kDa erythrocyte type II transmembrane protein that mediates calcium-dependent membrane phospholipids redistribution, PL Scramblase 1 is the protein product encoded by the founding member of the PLSCR family of genes (PLSCR1-5). All PLSCR family members, with the exception of PLSCR2, possess a proline-rich N-terminal region containing PxxP and PPxY domains, a cysteine-rich region, a conserved calcium-binding domain (EF-hand-like), and a putative transmembrane region enriched in hydrophobic amino acids. In addition, PLSCR1 contains a nuclear localization signal (NLS) and a DNA-binding domain that are essential for its nuclear localization and associated nuclear function. In addition to PLSCRs, TMEM16 and XKR family members have also been reported to mediate scramblase activity.

Specifications

• Antigen: PL Scramblase 1
• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 4D2
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Accession No.: O15162
• Gene Symbols: PLSCR1
• Host Species: Mouse
• Immunogen: Recombinant protein corresponding to human PL Scramblase 1.
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_066928
• Target Species: Human
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only