MilliporeSigma™ Mouse anti-Polycystin-1, Clone: E4 (5F4D2),

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1252

Catalog No. MABS1252

Description

Specifically detects Polycystin-1 clone: E4 (5F4D2) in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blotting

Polycystin-1 (UniProt P98161; also known as Autosomal dominant polycystic kidney disease 1 protein) is encoded by the PKD1 gene (Gene ID 5310) in human. The primary cilium is a microtubule-based mechano- and chemo-sensory organelle that coordinates an array of cellular pathways during development and in tissue homeostasis, including Hedgehog (Hh), PDGFRα and Wnt signaling. Defective ciliary function causes a variety of ciliopathies, including autosomal dominant polycystic kidney disease (ADPKD). Polycystin-1 (PC1) and polycystin-2 (PC2) are large transmembrane proteins co-localized to primary cilium, where they play an important role in calcium-based signalling. PC1 is an atypical adhesion G-protein-coupled receptor (aGPCR) with 11-transmemrbane domains. PC1 is cis-autoproteolytically cleaved at a juxtamembrane GPCR cleavage site (GPS; a.a. 3012-3061) in a developmental stage-dependent manner, where PC1 is largely uncleaved in early embryonic kidneys but becomes extensively cleaved after birth. GPS cleavage results in a heterodimeric PC1 form, in which the N-terminal fragment (NTF) remains non-covalently associated with the transmembrane C-terminal fragment (CTF). Transgenic mice expressing non-cleavable PC1 develop cystic kidney disease during the postnatal period.

Specifications

• Antigen: Polycystin-1
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: PKD1
• Immunogen: FLAG-tagged recombinant human polycystin-1 C-type lectin domain.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_001009944.2
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG1 κ

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: E4 (5F4D2)
• Gene Accession No.: P98161
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Purified

For Research Use Only