PRKDC, Mouse, Clone: 18-2, Abnova™

Catalog No.	Quantity
89-111-413	100 μg

Catalog No. 89-111-413 Supplier Abnova Corporation Supplier No. MAB3430

Description

Mouse monoclonal antibody raised against native PRKDC.

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM

Specifications

• Antigen: PRKDC
• Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Classification: Monoclonal
• Clone: 18-2
• Conjugate: Unconjugated
• Description: Mouse monoclonal antibody raised against native PRKDC.
• Dilution: Western Blot (1-2 ug/mL) The optimal working dilution should be determined by the end user.
• Formulation: In 10mM PBS, pH 7.4 (0.09% sodium azide)
• Gene: PRKDC
• Gene Alias: DNA-PKcs/DNAPK/DNPK1/HYRC/HYRC1/XRCC7/p350
• Gene Symbols: PRKDC
• Host Species: Mouse
• Immunogen: Native purified PRKDC from HeLa cells.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 5591
• Test Specificity: Reacts with the 460 KDa catalytic subunit of DNA-dependent Protein Kinase (DNA-PKcs). Inhibits kinase activity of DNA-PKcs ∼50%, but preincubation of enzyme with DNA protects DNA-PKcs from inactivation by clone 18-2. In Western blot, clone 18-2 reacts with intact DNA-PKcs as well as its degradation products.
• Target Species: Human, Rat
• Content And Storage: Store at 4°C.
• Form: Liquid
• Isotype: IgG1