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CD32 Mouse anti-Rat, Brilliant Violet 711, Clone: D34-485, BD Optibuild™
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The D34-485 monoclonal antibody specifically recognizes CD32, the Fc γII receptor. Rat CD32 is expressed on B lymphocytes, myeloid cells, and some lymphocytes in the thymic medulla. D34-485 antibody blocks binding of aggregated immunoglobulins to the Fc γII receptors in vitro.
The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Specifications
Specifications
| Antigen | CD32 |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | D34-485 |
| Concentration | 0.2mg/mL |
| Conjugate | Brilliant Violet 711 |
| Description | Fcgr2b; FcRII; Fc-gamma RII; FcγRII; FcγII receptor |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Host Species | Mouse |
| Immunogen | Recombinant Rat CD32 Protein |
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Safety and Handling
For Research Use Only
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