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CD4 Mouse anti-Rat, BUV395, Clone: OX-35, BD Optibuild™
Mouse Monoclonal Antibody
Supplier: BD Biosciences 740256
Description
The OX-35 clone recognizes the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, some dendritic cells, and microglia. CD4 is an antigen coreceptor on the T-cell surface that interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through it's association with the T-cell receptor complex and protein tyrosine kinase Lck. The OX-35 clone has been reported to bind to a different epitope of CD4 than that recognized by the W3/25 and OX-38 clones.
The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.
Specifications
CD4 | |
Monoclonal | |
0.2mg/mL | |
Cd4; CD4 antigen; p55; W3/25 antigen; T-cell surface glycoprotein CD4 | |
Mouse | |
Affinity Purified | |
RUO | |
Rat | |
IgG2a κ |
Flow Cytometry | |
OX-35 | |
Brilliant Ultraviolet 395 | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
Rat T-cell blasts | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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