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CD4 Mouse anti-Rat, Brilliant Violet 650, Clone: OX-35, BD Optibuild™
Mouse Monoclonal Antibody
Supplier: BD Biosciences 740513
Description
The OX-35 clone recognizes the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, some dendritic cells, and microglia. CD4 is an antigen coreceptor on the T-cell surface that interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through it's association with the T-cell receptor complex and protein tyrosine kinase Lck. The OX-35 clone has been reported to bind to a different epitope of CD4 than that recognized by the W3/25 and OX-38 clones.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
Specifications
CD4 | |
Monoclonal | |
0.2mg/mL | |
Cd4; CD4 antigen; p55; W3/25 antigen; T-cell surface glycoprotein CD4 | |
Mouse | |
Affinity Purified | |
RUO | |
Rat | |
IgG2a κ |
Flow Cytometry | |
OX-35 | |
Brilliant Violet 650 | |
Aqueous buffered solution containing ≤0.09% sodium azide. | |
Rat T-cell blasts | |
50 μg | |
Primary | |
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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