CD45 Mouse anti-Rat, BUV395, Clone: OX-1, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 740258

Catalog No. BDB740258

Description

The OX-1 antibody recognizes all molecular forms of CD45 (Leukocyte Common Antigen) on all hematopoietic cells except erythrocytes. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family; its intracellular (COOH-terminal) region contains two PTP catalytic domains while the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), and differing levels of glycosylation. The CD45 isoforms detected in rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.

Specifications

• Antigen: CD45
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: Ptprc; Lca; Leucocyte common antigen; RT7; T200
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Rat
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: OX-1
• Conjugate: Brilliant Ultraviolet 395
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Leukocyte Common Antigen-enriched Glycoprotein Fraction from Wistar Rat Thymocytes
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

For Research Use Only