CD45RA Mouse anti-Rat, Brilliant Violet 605, Clone: OX-33, BD Optibuild™

Catalog No.	Quantity
BDB740372	50 μg

Catalog No. BDB740372 Supplier BD Biosciences Supplier No. 740372

Description

Mouse Monoclonal Antibody

The OX-33 antibody specifically recognizes a high-molecular-weight form of CD45 found only on B lymphocytes. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.

Specifications

• Antigen: CD45RA
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: OX-33
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 605
• Description: Ptprc; Lca; Leucocyte common antigen
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Immunogen: Leukocyte common antigen purified from rat splenocytes
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Rat
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG1 κ

For Research Use Only