MilliporeSigma™ Mouse anti-Serotonin receptor 2A/HTR2A, Clone: 9B11.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABN1595

Catalog No. MABN1595

Description

Specifically detects Serotonin receptor 2A/HTR2A clone: 9B11.1 in Human, Mouse, Rat samples, and it is validated for Immunohistochemistry, Western Blotting

5-hydroxytryptamine receptor 2A (UniProt P28223; also known as 5-HT-2, 5-HT-2A, 5-HT2A, Serotonin receptor 2A) is encoded by the HTR2A (also known as HTR2) gene (Gene ID 3356) in human. Hydroxytryptamine (5-HT) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals. In the periphery, 5-HT mediates gastrointestinal motility, platelet aggregation, and contraction of blood vessels. Many functions of the central nervous system are influenced by 5-HT, including sleep, motor activity, sensory perception, arousal and appetite. A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT. 5-HT2A receptor, which couples to Gq/11 to increase intracellular calcium, is widely expressed at central and peripheral sites of 5-HT action and contributes to many of the physiological effects of 5-HT. The hallucinogenic activity of LSD is mediated in part by its action as a partial to full agonist of 5-HT2A receptor, and the activity of atypical antipsychotics such as clozapine appears to be mediated in part by antagonism against 5-HT2A receptor. Human 5-HT2A receptor contains four extracellular domains (a.a. 1-75, 133-148, 216-233, 347-362), seven transmembrane segments (a.a. 76-99, 111-132, 149-171, 192-215, 234-254, 325-346, 363-384), and four cytoplasmic domains (a.a. 100-110, 172-191, 255-324, 385-471).

Specifications

• Antigen: Serotonin receptor 2A/HTR2A
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: HTR2A; HTR2
• Immunogen: GST-tagged recombinant fragment corresponding to the third cytoplasmic domain of human Serotonin receptor 2A/HTR2A.
• Quantity: 100 μg
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_000612
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG2a κ

• Applications: Immunohistochemistry, Western Blot
• Clone: 9B11.1
• Gene Accession No.: P28223
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Purified

For Research Use Only