MilliporeSigma™ Mouse anti-SPOP, Clone: 9B7.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABC565

Catalog No. MABC565MI

Description

Specifically detects SPOP clone: 9B7.1 in Human samples, and it is validated for Immunohistochemistry, Western Blotting

Speckle-type pox virus and zinc finger (POZ) protein, also known as HIB homolog 1 or Roadkill homolog 1, is encoded by the SPOP gene (also known as TEF2 or BTBD32) in human (Entrez Gene ID 8405). SPOP protein is a component of cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex and mediates recruitment of target proteins containing the SPOP-binding consensus (SBC) motif. The 42kDa SPOP protein comprises an N-terminal MATH domain, a bric-a-brac, tramtrack and broad complex (BTB)/POZ domain, a 3-box domain, and a C-terminal nuclear localization sequence. The MATH domain is primarily involved in substrate recognition/binding. Known substrates with SBC motifs include Macro H2A, Puc (Puckered), Daxx (Death domain–associated protein), and Gli (Glioma-associated oncogene). Prostate and endometrial cancers are frequently identified with SPOP mutations in the meprin and TRAF (Tumor necrosis factor receptor-associated factor) homology (MATH) domain, which can potentially lead to altered substrate binding activities. (PMID 25058385). The SPOP protein sequence is 100% conserved among human, murine, and rat (UniProt O43791, Q6ZWS8, and B2RYD9, respectively) species. Anti-SPOP, clone 9B7.1, is a purified mouse monoclonal antibody. A GST fusion protein containing the full-lenght human SPOP sequence was used as the immunogen. The binding epitope of clone 9B7.1 has not yet been mapped.

Specifications

• Antigen: SPOP
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG2aκ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: SPOP
• Immunogen: GST-tagged recombinant protein corresponding to human SPOP.
• Quantity: 100 μg
• Research Discipline: Apoptosis, Cancer
• Gene ID (Entrez): NP_001007227
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG2a κ

• Applications: Immunohistochemistry, Western Blot
• Clone: 9B7.1
• Gene Accession No.: O43791
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

For Research Use Only