MilliporeSigma™ Mouse anti-Src, Clone: GD11, Alexa Fluor 488,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ 05184AF488

Catalog No. 05-184-AF488

Description

Specifically detects Src clone: GD11 in Avian, Human, Mink, Mouse, Rat samples, and it is validated for Immunocytochemistry

Tyrosine-protein kinase transforming protein Src (EC 2.7.10.2; UniProt P00526; also known as p60-Src, pp60v-src, v-Src) is encoded by the V-SRC gene (Gene ID 1491925) of Rous sarcoma virus strain Prague C (RSV-PrC). Rous sarcoma virus (RSV) V-SRC gene is the first retroviral oncogene discovered. V-SRC encodes the v-Src tyrosine kinase that transforms infected host cells into uncontrolled mitosis, providing abundant cells for fresh infection. The non-oncogenic chicken c-Src (cellular Src) gene structurally related to v-Src was discovered later in 1979 by J. Michael Bishop and Harold E. Varmus. It is believed that RSV originally took up the chicken proto-oncogene SRC (Gene ID 396442; UniProt P00523) and incorporated into its genome in the mutated concogene form. c-Src contains six functional regions, Src homology 4 (SH4) domain, unique region, SH3 domain, SH2 domain, catalytic domain, and short regulatory tail. In its inactive state, Src is phosphorylated at Tyr527. The pTyr527 interacts with the SH2 domain which helps the SH3 domain interact with the linker domain and consequently keeps the inactive unit tightly bound. Dephosphorylation of pTyr527 destabilizes the inactive conformation, opening up the SH3, SH2 and the kinase domains, leading to Tyr416 autophosphorylation and c-Src activation.

Specifications

• Antigen: Src
• Classification: Monoclonal
• Conjugate: Alexa Fluor 488
• Gene Accession No.: P00526
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Avian, Human, Mink, Mouse, Rat
• Form: Purified

• Applications: Immunocytochemistry
• Clone: GD11
• Formulation: Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15mg/mL BSA and 0.1% sodium azide.
• Gene Symbols: V-SRC; SRC
• Immunogen: Recombinant PrA-RSV p60src.
• Quantity: 100 μL
• Research Discipline: Cell Structure
• Gene ID (Entrez): P00526.3
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG1

For Research Use Only