MilliporeSigma™ Mouse anti-Thymidylate Kinase/TMPK, Clone: 1A5.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABC609

Catalog No. MABC609MI

Description

Specifically detects Thymidylate Kinase/TMPK clone: 1A5.1 in Human samples, and it is validated for Immunohistochemistry (Paraffin), Western Blotting

Thymidylate kinase (EC 2.7.4.9; UniProt P23919; also known as dTMP kinase) is encoded by the DTYMK (also known as CDC8, PP3731, TMPK, TYMK) gene (Gene ID 1841) in human. Thymidylate kinase (TMPK) is an ubiquitously expressed enzyme of the dTTP synthesis pathway involved in DNA synthesis. TMPK catalyzes the phosphorylation of thymidine 5'-monophosphate (dTMP) in the presence of ATP and Mg²⁺ to form thymidine 5'-diphosphate (dTDP), which is then further phosphorylated by nucleoside-diphosphate kinase (NDK) to thymidine 5'-triphosphate (dTTP). Aside from its normal physiological role, TMPK activity is essential for the activation of nucleoside analogs-based prodrugs, such as the clinically used anti-HIV prodrugs AZT and d4T, which need to be converted to the triphosphate forms for their pharmacological activities. Inhibition of TMPK activity is reported to augment the potency of anticancer agent doxorubicin against human colon cancer cells regardless of their p53 status. In addition, lung cancers with LKB1-mutations are reported to show deficits in nucleotide metabolism and exhibit hypersensitivity to DTYMK inhibition.

Specifications

• Antigen: Thymidylate Kinase/TMPK
• Classification: Monoclonal
• Formulation: Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Symbols: DTYMK; CDC8; TMPK; TYMK
• Immunogen: GST-tagged recombinant protein corresponding to human Thymidylate Kinase/TMPK.
• Quantity: 100 μg
• Research Discipline: Apoptosis, Cancer
• Gene ID (Entrez): NP_001158503
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Isotype: IgG2a κ

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Clone: 1A5.1
• Gene Accession No.: P23919
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified

For Research Use Only