TIGIT Mouse anti-Mouse, APC-R700, Clone: 1G9, BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 565474

Catalog No. BDB565474

Description

The 1G9 monoclonal antibody specifically binds to T cell immunoreceptor with Ig and ITIM domains (TIGIT) which is also known as, V-set and transmembrane domain-containing protein 3 (Vstm3). TIGIT is a 26 kDa, type I transmembrane glycoprotein that belongs to the poliovirus receptor (PVR) family. TIGIT is expressed on activated and memory CD4+ T cells and CD8+ T cells, follicular T helper cells, regulatory T cells, and NK cells. Its ligands include the poliovirus receptor (PVR, aka, CD155), poliovirus receptor-related protein 2 (PVRL2, aka, CD112), and poliovirus receptor-related protein 3 (PVRL3, CD113). TIGIT functions as a negative regulator of T cell and NK cell responses including proliferation, cytotoxicity, and the production of proinflammatory cytokines. TIGIT-deficient mice are reportedly more susceptible to developing autoimmune diseases in certain model systems.

This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor™ 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor™ (eg, 730/45-nm filter).

Specifications

• Antigen: TIGIT
• Clone: 1G9
• Conjugate: APC-R700
• Formulation: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
• Host Species: Murine
• Isotype: IgG₁, κ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal

• Applications: Flow Cytometry
• Concentration: 0.2mg/mL
• Description: Tigit; Vstm3; T cell immunoreceptor with Ig and ITIM domains
• Format: Affinity Purified
• Immunogen: Mouse TIGIT Recombinant Protein
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine