TLR4 (CD284) Mouse anti-Human, Brilliant Violet 711, Clone: TF901, BD
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 564404
The TF901 monoclonal antibody specifically binds to TLR4 (Toll-like receptor 4) which is also known as CD284. TLR4 is a 110 kDa type I transmembrane glycoprotein that belongs to the Toll-like receptor (TLR) family. TLR4 is expressed on monocytes, macrophages, granulocytes, dendritic cells and endothelial cells. TLR4 binds lipopolysaccharide (LPS) which is a major membrane component of Gram-negative bacteria. It thus acts as an innate immune recognition receptor against many pathogens. In association with MD-2, LPS binds to and signals through TLR4 receptors by MyD88-dependent and MyD88-independent pathways to induce the cellular production of proinflammatory cytokines. TF901 antibody binding is not influenced by the human TLR4 D299G/T399I polymorphism. Preincubation of TLR4-positive cells with the TF901 antibody blocks the subsequent binding of the HTA125 antibody which is also specific for human TLR4.
The antibody was conjugated to BD Horizon BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|TLR4; CD284; Toll-like Receptor 4; ARMD10|
|Human TLR4 Transfected Cell Line|
|Brilliant Violet 711|
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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