Mouse anti-TNF-alpha, Clone: P/T2, Azide Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP23470302	Novus Biologicals NBP2347030.2 MG	0.2 mg

Description

TNF-alpha Monoclonal antibody specifically detects TNF-alpha in Human, Mouse, Rat, Canine, Feline, Rabbit, Zebrafish samples. It is validated for Flow Cytometry, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, CyTOF-ready.

Specifications

• Antigen: TNF-alpha
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Asthma, Autophagy, Biologically Active Proteins, Cancer, Cytokine Research, Diabetes Research, Immune System Diseases, Immunology, Innate Immunity
• Formulation: 10mM PBS - BSA free with No Preservative
• Gene Alias: APC1 protein, Cachectin, DIF, TNF, monocyte-derived, TNF-a, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Gene Symbols: TNF
• Isotype: IgM κ
• Purification Method: Protein A purified
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 17 kDa

• Clone: P/T2
• Dilution: Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein, Immunohistochemistry-Paraffin 1-2ug/ml, Immunohistochemistry-Frozen 1-2ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Canine, Feline, Rabbit, Zebrafish
• Gene Accession No.: P01375
• Gene ID (Entrez): 7124
• Immunogen: A hexadecapeptide corresponding to aa115-130 of human TNF-alpha, conjugated to thyroglobulin
• Primary or Secondary: Primary
• Reconstitution: Protein A purified
• Test Specificity: Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.

For Research Use Only