MilliporeSigma™ Mouse anti-Vinculin (Ascites Free), Clone: VIIF9 (7F9),

Catalog No.	Quantity
MAB3574C	100 μg

Catalog No. MAB3574C Supplier MilliporeSigma™ Supplier No. MAB3574C

Description

Mouse Monoclonal Antibody

Specifically detects Vinculin (Ascites Free) clone: VIIF9 (7F9) in Human, Mouse, Rabbit, Rat samples, and it is validated for Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blotting

Metavinculin (MV) and its a.a. 916-983 truncated splice variant Vinculin (UniProt P18206) are cytoskeleton proteins encoded by the VCL (also known as MVCL, CMD1W, CMH15) gene (Gene ID 7414) in human. The N-terminal globular head domain (a.a. 2-835) of Vinculin/VM contains binding sites for talin and α-actinin as well as a tyrosine phosphorylation site, while their C-terminal Tail region (a.a. 879-1134 of VM; a.a. 879-1066 of Vinculin) contains binding sites for F-actin, paxillin, and lipids. Vinculin is associated with focal adhesion and adherens junctions, which are complexes that nucleate actin filaments and crosslinkers between the external medium, plasma membrane, and actin cytoskeleton. The loss of vinculin disrupts focal adhesion complex formation and prevents cell adhesion and spreading, accompanied by reduced stress fiber formation and inhibition of lamellipodia extension. Smooth muscles and skeletal muscles in their well-differentiated, contractile state co-express vinculin and meta-vinculin. Both vinculin isoforms co-localize in muscular adhesive structures, such as dense plaques in smooth muscles, intercalated discs in cardiomyocytes, and costameres in skeletal muscles.

Specifications

• Antigen: Vinculin (Ascites Free)
• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: VIIF9 (7F9)
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
• Gene Accession No.: P18206
• Gene Symbols: VCL; MVCL; CMD1W; CMH15
• Host Species: Mouse
• Immunogen: Human uterus meta-vinculin.
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003364
• Target Species: Human, Mouse, Rabbit, Rat
• Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only