MilliporeSigma™ Mouse anti-XPB, Clone: 15TF2-1B3,

Catalog No.	Quantity
MABE1123MI	100 μL

Catalog No. MABE1123MI Supplier MilliporeSigma™ Supplier No. MABE1123

Description

Mouse Monoclonal Antibody

Specifically detects XPB clone: 15TF2-1B3 in Human samples, and it is validated for Immunocytochemistry, Western Blotting

Transcription factor II human (TFIIH) basal transcription factor complex helicase XPB subunit (EC 3.6.4.12; UniProt P19447; also known as BTF2 p89, DNA excision repair protein ERCC-3, DNA repair helicase, DNA repair protein complementing XP-B cells, TFIIH 89kDa subunit, TFIIH basal transcription factor complex 89kDa subunit, TFIIH p89, Basic transcription factor 2 89kDa subunit, Xeroderma pigmentosum group B-complementing protein) is encoded by the ERCC3 (also known as BTF2, GTF2H, RAD25, TFIIH, XPB) gene (Gene ID 2071) in human. DNA lesions caused by UV irradiation, drugs, or other environmental factors are eliminated by two nucleotide excision repair (NER) pathways, Global genome repair (GGR) and transcription-coupled repair (TCR). In GGR, the removal of lesions requires their recognition by the repair factor XPC/HR23b and the subsequent opening of the DNA duplex by TFIIH. The resulting single-stranded structure is stabilized by XPA and replication protein A (RPA). XPG is recruited through its interaction with TFIIH on the 3′ side of the lesion and its positioning on the cut site requires RPA. The interaction between XPA and XPB (ERCC1) stimulates the recruitment of ERCC1-XPF on the 5′ side of the DNA lesion. The damaged oligonucleotide can then be removed through the double incision by XPG and ERCC1-XPF endonucleases. In TCR, these factors (except XPC/HR23B) are recruited by the stalled RNA pol II in front of the damage with the help of the CSB and CSA proteins.

Specifications

• Antigen: XPB
• Applications: Immunocytochemistry, Western Blot
• Classification: Monoclonal
• Clone: 15TF2-1B3
• Formulation: Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
• Gene Accession No.: P19447
• Gene Symbols: ERCC3; XPB; XPBC
• Host Species: Mouse
• Immunogen: Recombinant protein corresponding to human XPB.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Epigenetics and Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_000113
• Target Species: Human
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
• Form: Ascites
• Isotype: IgG1 κ

For Research Use Only