MilliporeSigma™ Mouse anti-XPB, Clone: 15TF2-1B3,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABE1123

Catalog No. MABE1123MI

Description

Specifically detects XPB clone: 15TF2-1B3 in Human samples, and it is validated for Immunocytochemistry, Western Blotting

Transcription factor II human (TFIIH) basal transcription factor complex helicase XPB subunit (EC 3.6.4.12; UniProt P19447; also known as BTF2 p89, DNA excision repair protein ERCC-3, DNA repair helicase, DNA repair protein complementing XP-B cells, TFIIH 89kDa subunit, TFIIH basal transcription factor complex 89kDa subunit, TFIIH p89, Basic transcription factor 2 89kDa subunit, Xeroderma pigmentosum group B-complementing protein) is encoded by the ERCC3 (also known as BTF2, GTF2H, RAD25, TFIIH, XPB) gene (Gene ID 2071) in human. DNA lesions caused by UV irradiation, drugs, or other environmental factors are eliminated by two nucleotide excision repair (NER) pathways, Global genome repair (GGR) and transcription-coupled repair (TCR). In GGR, the removal of lesions requires their recognition by the repair factor XPC/HR23b and the subsequent opening of the DNA duplex by TFIIH. The resulting single-stranded structure is stabilized by XPA and replication protein A (RPA). XPG is recruited through its interaction with TFIIH on the 3′ side of the lesion and its positioning on the cut site requires RPA. The interaction between XPA and XPB (ERCC1) stimulates the recruitment of ERCC1-XPF on the 5′ side of the DNA lesion. The damaged oligonucleotide can then be removed through the double incision by XPG and ERCC1-XPF endonucleases. In TCR, these factors (except XPC/HR23B) are recruited by the stalled RNA pol II in front of the damage with the help of the CSB and CSA proteins.

Specifications

• Antigen: XPB
• Classification: Monoclonal
• Formulation: Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
• Gene Symbols: ERCC3; XPB; XPBC
• Immunogen: Recombinant protein corresponding to human XPB.
• Quantity: 100 μL
• Research Discipline: Epigenetics and Nuclear Function
• Gene ID (Entrez): NP_000113
• Content And Storage: Stable for one year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles which may damage IgG and affect product performance.
• Isotype: IgG1 κ

• Applications: Immunocytochemistry, Western Blot
• Clone: 15TF2-1B3
• Gene Accession No.: P19447
• Host Species: Mouse
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Ascites

For Research Use Only