ZAP70, Mouse, Clone: ZAP-03, Abnova™

Catalog No.	Quantity
89-111-518	100 μg

Catalog No. 89-111-518 Supplier Abnova Corporation Supplier No. MAB3664

Description

Mouse monoclonal antibody raised against partial recombinant ZAP70.

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq

Specifications

• Antigen: ZAP70
• Applications: Flow Cytometry, Immunocytochemistry, Western Blot
• Classification: Monoclonal
• Clone: ZAP-03
• Conjugate: Unconjugated
• Description: Mouse monoclonal antibody raised against partial recombinant ZAP70.
• Dilution: Flow Cytometry (2-5 ug/mL) Western Blot (0.5 ug/mL) The optimal working dilution should be determined by the end user.
• Formulation: In PBS, pH 7.4 (0.09% sodium azide)
• Gene: ZAP70
• Gene Alias: FLJ17670/FLJ17679/SRK/STD/TZK/ZAP-70
• Gene Symbols: ZAP70
• Host Species: Mouse
• Immunogen: Recombinant His fusion protein corresponding to 160 amino acids of human ZAP70.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 7535
• Test Specificity: This antibody reacts with ZAP70, a 70 KDa protein tyrosine kinase expressed in T and NK cells. ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
• Target Species: Human
• Content And Storage: Store at 4°C. Do not freeze.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid
• Isotype: IgG1