ZAP70, Mouse, Clone: ZAP-03, Abnova™

Mouse monoclonal antibody raised against partial recombinant ZAP70.

Supplier:  Abnova Corporation MAB3664

Catalog No. 89-111-518

Description

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq

Specifications

• Antigen: ZAP70
• Classification: Monoclonal
• Conjugate: Unconjugated
• Dilution: Flow Cytometry (2-5 ug/mL) Western Blot (0.5 ug/mL) The optimal working dilution should be determined by the end user.
• Gene: ZAP70
• Gene Symbols: ZAP70
• Immunogen: Recombinant His fusion protein corresponding to 160 amino acids of human ZAP70.
• Regulatory Status: RUO
• Gene ID (Entrez): 7535
• Target Species: Human
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry, Western Blot
• Clone: ZAP-03
• Description: Mouse monoclonal antibody raised against partial recombinant ZAP70.
• Formulation: In PBS, pH 7.4 (0.09% sodium azide)
• Gene Alias: FLJ17670/FLJ17679/SRK/STD/TZK/ZAP-70
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: This antibody reacts with ZAP70, a 70 KDa protein tyrosine kinase expressed in T and NK cells. ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
• Content And Storage: Store at 4°C. Do not freeze.
  Aliquot to avoid repeated freezing and thawing.
• Isotype: IgG1