Mouse CD3 epsilon Antibody, R&D Systems™

Hamster Monoclonal Antibody has been used in 14 publications

Catalog Number	Mfr. No.	Quantity
MAB484SP	R&D Systems MAB484SP	25 μg
MAB484100	R&D Systems MAB484100	100 μg
MAB484500	R&D Systems MAB484500	500 μg

Description

CD3 epsilon Monoclonal specifically detects CD3 epsilon in Mouse samples. It is validated for Flow Cytometry, Immunoprecipitation, Cell depletion, T Cell Stimulation, CyTOF-reported.

Specifications

• Antigen: CD3 epsilon
• Dilution: Flow Cytometry 2.5 ug/10^6 cells, Immunoprecipitation, Cell depletion, T Cell Stimulation 0.1-0.6 ug/mL, CyTOF-reported
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene ID (Entrez): 916
• Immunogen: H-2K b -specific mouse cytotoxic T-lymphocyte
• Primary or Secondary: Primary
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Test Specificity: Detects mouse CD3 epsilon . Binds to lymphocytes from all mouse strains tested and does not react with cells from rats, rabbits, miniature swine, or hamsters. 1 Binds to the CD3 epsilon-chain present on T-lymphocyes and thymocytes. Its binding has been characterized with respect to several other monoclonal anti-CD3 antibodies. 3,4

• Clone: 145-2C11
• Applications: Flow Cytometry, Immunoprecipitation, Depletion Assay, T-Cell Activation, CyTOF
• Conjugate: Unconjugated
• Host Species: Hamster
• Target Species: Mouse
• Gene Alias: CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, FLJ18683, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE
• Gene Symbols: Cd247
• Isotype: IgG
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

For Research Use Only