R&D Systems™ Recombinant Mouse FAP Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2000 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: P97321
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Gene ID (Entrez): 2191
• Molecular Weight (g/mol): M.W. (Observed): 78-90 kDa, reducing conditions; M.W. (theoretical): 85 kDa
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived mouse Fibroblast Activation Protein alpha/FAP protein Leu26-Asp761
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: 170 kDa melanoma membrane-bound gelatinase, DKFZp686G13158, DPPIV, EC 3.4.21.-, FAP, FAPA, Fibroblast activation protein alpha, fibroblast activation protein, alpha, Integral membrane serine protease, seprase, vibronectin
• Common Name: Fibroblast Activation Protein alpha/FAP
• Gene Symbol: Fap
• Biological Activity: Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2000 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
• Conjugate: Unconjugated
• Species: Mouse
• Recombinant: Recombinant
• Expression System: Mouse myeloma cell line, NS0-derived Leu26-Asp761 Accession # P97321
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Fibroblast Activation Protein alpha/FAP