Invitrogen™ eBioscience™ Mouse GM-CSF ELISA Ready-SET-Go!™ Kit 

Specifically engineered for accurate and precise measurement of mouse GM-CSF protein levels from samples including serum, and cell culture supernatants.

Manufacturer:  Invitrogen™ 88733486

Catalog No. 50-173-42

Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer . Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme. Pre-titrated Avidin-HRP
Substrate. 1X TMB Solution

Description

GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombinant GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS).

Specifications

• Accession Number: P01587
• Conjugate: Biotin
• Gene ID (Entrez): 12981
• For Use With (Equipment): Colorimetric Microplate Reader
• Product Type: ELISA
• Regulatory Status: RUO
• Sample Type: Serum, Supernatants
• Species: Mouse
• Test Time: 1 hr 45 min
• Target: Mouse
• Synonym: Granulocyte-macrophage colony stimulating factor, Colony-stimulating factor, CSF12

• Assay Range: 4-500 pg/mL
• For Use With (Application): ELISA
• Includes: 96-well plates
• Kit Contents: 10X Coating Buffer: Buffer for plating the Capture Antibody
• Quantity: 10 x 96 tests
• Sensitivity: 4 pg/mL
• Sample Volume: Serum (100μL), Supernatants (100μL)
• Storage Requirements: 2-8°C
• Total Assay Time: 24 hr 30 min
• Gene Symbols: Csf2
• Label or Dye: HRP

For Research Use Only