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R&D Systems™ Recombinant Mouse Hepsin Protein

Catalog No. 9156SE010
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91-56S-E010 10 μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to cleave tert-butoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC). The specific activity is >8,000 pmol/min/μg, as measured under the described conditions.

Specifications

Accession Number O35453-2
For Use With (Application) Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Gene ID (Entrez) 3249
Molecular Weight (g/mol) M.W. (Observed): 41-47 kDa, reducing conditions; M.W. (theoretical): 42 kDa
Quantity 10 μg
Source Mouse myeloma cell line, NS0-derived mouse Hepsin protein Gln44-Pro416 (Asp160Glu & Arg161Lys), with a C-terminal 10-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Gene Alias EC 3.4.21, EC 3.4.21.106, hepsin, HPN, serine protease hepsin, serine protease hepsin catalytic chain, TMPRSS1 TMPRSS1serine protease hepsin non-catalytic chain, Transmembrane protease serine 1, transmembrane protease, serine 1
Common Name Hepsin
Biological Activity Measured by its ability to cleave tert-butoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC). The specific activity is >8,000 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on RnDSystems.com.
Conjugate Unconjugated
Species Mouse
Recombinant Recombinant
Expression System Mouse myeloma cell line, NS0-derived Gln44-Pro416 (Asp160Glu & Arg161Lys), with a C-terminal 10-His tag
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein Hepsin
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