Invitrogen™ Mouse IFNa ProQuantum Immunoassay Kit

Description

ELISA

Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.

• High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
• Broad dynamic range-≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
• Small sample consumption-use 2–5 μL of sample (compared to 75 μL for triplicate wells with other methods)
• Fast, easy workflow-2 hours from sample to answer, with no wash steps
• No proprietary instrument to purchase-runs on any real-time PCR instrument

IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.

Specifications

• Accession Number: alpha-2 A interferon; Gm12597; ifa; Ifa1; Ifa2; IFL; IFN; IFN alpha; IFN a; IFN1@; IFNA; IFNA@; IFNA1; IFNA13; Ifna15; IFNA2; IFNA2A; IFNA2B; IFNA2C; IFNA3; IFNA6; Ifnaa; IFNAalphaIFN-alpha-1/13; IFNalpha; IFN-alpha; IFN-alpha 1 b; IFN-alpha1; IFN-alpha-1; IFN-alpha-1/13; IFN-alpha-2; IFN-alpha-6; IFN-alphaA; IFN-alphaD; IFN-gamma; IFNa; INFA2; interferon; interferon alpha; interferon alpha 1; interferon alpha 15; interferon alpha 1 b; interferon alpha 2; interferon alpha 2 A; interferon alpha 2 b; interferon alpha A; interferon alpha family gene 1; interferon alpha family, gene 2; interferon alpha protein; interferon alpha-1; interferon alpha-1 precursor; Interferon alpha-1/13; interferon alpha-2; Interferon alpha-A; Interferon alpha-D; interferon type A1/A2; interferon type A3; interferon, alpha 1; interferon, alpha 2; interferon-alpha; interferon-alpha 1; interferon-alpha1; interferon-alpha-6; leIF A; LeIF D; MGC138207; MGC138505; MGC138507; MuIFN-alpha-A; novel interferon alpha (Ifna) family member; OTTMUSG00000007655
• Assay Range: 1.6 to 25,000 pg/mL
• Assay Sensitivity: <1 pg/mL
• Conjugate: FAM™
• Product Type: ProQuantum
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): qPCR Instruments
• Gene ID (Entrez): 111654, 15962, 15965, 242517
• Gene Symbol: IFNA, IFNA1, Ifna15, IFNA2
• Kit Contents: Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
• Storage Requirements: -20°C
• Target: IFN alpha
• Test Time: 30 mins
• Total Assay Time: 2 hrs