Mouse IL-10 ELISA Standard,Invitrogen
Manufacturer: Invitrogen 29810165
DescriptionInterleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract. Description: Mouse IL-10 is an ~18 kDa cytokine, also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response. Applications Reported: The Mouse IL-10 Lyophilized Standard is intended to be used as a standard for the Mouse IL-10 Ready-Set-Go! ELISA kit (cat. 88-7105). Typically the standard is run in a series of 7 doubling dilutions from the ft.top ft. standard. Please refer to the Certificate of Analysis included with each ELISA set for instructions on the proper reconstitution and dilution of the lyophilized standard. Note: These are single-use vials. Use one time within 4 hours of reconstitution and discard. Reconstitute and dilute as suggested in the Certificate of Analysis or recovery may not be accurate. Applications Tested: The Mouse IL-10 Lyophilized Standard has been calibrated and tested in the Mouse IL-10 Ready-Set-Go! ELISA kit (cat. 88-7105). Source: E. coli expressed amino acids Ser 19-Ser 178 of mature mouse IL-10 accession &Num; NM_010548 with a Cys149tyr substitution.
|E. coli-expressed amino acids ser 19-ser 178 of mature mouse IL-10 (accession # NM_010548) with a cys149tyr substitution (Moore, K. et al., Science 248:1230-1234).|
|Mouse IL-10 ELISA Standard|
|protein with no preservative|
|Mouse IL-10 ELISA Standard|
Safety and Handling
ShelfLife : 12 Months
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