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Invitrogen™ IL-28B/(IFN lambda 3) Mouse Uncoated ELISA Kit with Plates Non-distribution product as customer accommodation.

Uncoated ELISA Kit with Plates

Manufacturer:  Invitrogen™ 88728422

Catalog No. 50-112-5198


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Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer. 10X PBS ELISA coating buffer
Assay Diluent. 5X concentrated
Detection Enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Solution
Certificate of Analysis. Lot-specific instructions for the dilution of antibodies and standards

Description

Description

The Mouse Interleukin 28B (IFN lambda 3) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-28B. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

The interleukins are a broad family of well characterized cytokines, primarily of hematopoietic cell origin. As new cytokines are molecularly characterized, they are assigned an IL number to maintain a standard nomenclature. The interleukins are secreted by immune cells (mainly macrophages, B-cells or T-cells) that regulate a wide range of immune system functions. The functions of different interleukins vary from the regulation of inflammatory and immune responses to the regulation of other interleukins. IL-28 and IL-29 are induced by viral infection and show antiviral activity. IL-28 may play a role in antiviral immunity through up-regulation of MHC class I antigen expression. IL-28 is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA.
Specifications

Specifications

Q8CGK6
Biotin
338374
96-well Plates: Corning Costar flat-bottom plates (included with Cat. Nos. ending in -22 and -86)
2 x 96 tests
32 pg/mL
Serum,100 μL|Supernatant,100 μL
2-8°C
24 hr 30 min
Ifnl3
HRP
32-4,000 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
RUO
Serum,Supernatant
Mouse
1 hr 45 min
Mouse
Interleukin-28B
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