Invitrogen™ IL-33 Mouse Uncoated ELISA Kit with Plates

Specifically engineered for precise measurement of mouse IL-33 protein levels from samples, including serum, and cell culture supernatants.

Catalog Number	Mfr. No.	Quantity
50-112-5200	Invitrogen™ 88733386	10 x 96 Tests
50-112-8739	Invitrogen™ 88733322	2 x 96 Tests

Description

The Mouse Interleukin 33 (IL-33) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-33. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Mouse IL-33 ELISA Ready-SET-Go! reagent set (with or without high affinity-binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA)

Specifications

• Accession Number: Q8BVZ5
• Assay Sensitivity: 25 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL-1,IL1-BETA,IL1F2
• Label or Dye: HRP
• Sample Volume: Serum, 100 μL; Supernatant, 100 μL
• Synonym: Interleukin-33
• Test Time: 1 hr. 45 min.

• Assay Range: 25-3,000 pg/mL
• Conjugate: Biotin
• Sample Type: Serum, Supernatant
• For Use With (Application): ELISA
• Gene ID (Entrez): 77125
• Kit Contents: Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, Coating Buffer: 10X PBS ELISA Coating Buffer, Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Streptavidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: NUNC Maxisorp flat-bottom (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 24 hr. 30 min.

For Research Use Only