Gibco™ Mouse LIF, Animal-Free Recombinant Protein, PeproTech®

Description

Recombinant Protein

Recombinant Murine LIF is a 19.9 kDa protein containing 180 amino acids residues, including three disulfide bonds. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family. LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue. LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity. However, LIF inhibition of stem cell differentiation appears to be mouse-specific.

Specifications

• Accession Number: P09056
• For Use With (Application): Functional Assay
• Format: Lyophilized
• Formulation: protein with no preservative
• Gene ID (Entrez): 16878
• Molecular Weight (g/mol): 19.6 kDa
• Name: Mouse LIF, Animal-Free
• Purification Method: HPLC, SDS-PAGE
• Quantity: 5 μg
• Regulatory Status: RUO
• Endotoxin Concentration: <0.1 EU/ μg
• Gene Alias: CDF; cholinergic differentiation factor; cholinergic neuronal differentiation factor; d factor; DIA; differentiation inhibitory activity; differentiation-inducing factor; differentiation-stimulating factor; Emfilermin; hepatocyte-stimulating factor III; HILDA; human interleukin in DA cells; leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); LIF; Melanoma-derived LPL inhibitor; MLPLI; myeloid leukaemia inhibitory factor
• Common Name: LIF
• Gene Symbol: Lif
• Biological Activity: PeproTech's Murine LIF is fully biologically active when compared to standards. The ED50 as determined by the M1 cell differentiation assay is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: SPLPITPVNA TCAIRHPCHG NLMNQIKNQL AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK QVISVVVQAF
• Content And Storage: -20°C
• Activity: PeproTech's Murine LIF is fully biologically active when compared to standards. The ED50 as determined by the M1 cell differentiation assay is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10^7 units/mg
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.