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Gibco™ Mouse M-CSF Recombinant Protein

Catalog No. PMC2044
Encompass_Preferred
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Quantity:
10 μg

Recombinant Protein

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, distilled water, to a concentration of 0.1-0.5 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -80°C to -20°C. Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 3 months at -80°C to -20°C. Avoid repeated freeze/thaw cycles.

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P07141
For Use With (Application) Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot
Formulation 5 mM tris with 100 mM NaCl and no preservative
Gene ID (Entrez) 12977
Molecular Weight (g/mol) 36.4 kDa
Name Mouse M-CSF
Purification Method Sequential Chromatography
Quantity 10 μg
Endotoxin Concentration <0.1 ng/ μg
Gene Alias C87615; colony stimulating factor 1; colony stimulating factor 1 (macrophage); colony-stimulating factor-1 splice variant; CSF1; CSF-1; Csfm; H-MCSF; lanimostim; macrophage colony stimulating factor 1; macrophage colony stimulating factor alpha; macrophage colony stimulating factor beta; macrophage colony-stimulating factor; macrophage colony-stimulating factor 1; macrophage colony-stimulating factor beta; macrophage-colony stimulating factor alpha; MCSF; MCSF alpha; MCSFBETA; MGC31930; M-MCSF; op; osteopetrosis; Processed macrophage colony-stimulating factor 1
Common Name M-CSF
Biological Activity ED50 ≤ 1.0 ng/mL; determined by the dose-dependent proliferation of M-NFS-60 cells.
Conjugate Unconjugated
Species Mouse
Recombinant Recombinant
Sequence Mouse M-CSF recombinant protein is a homodimer consisting of two 156 amino acid subunits
Content And Storage -20°C
Endotoxin Level <0.1 ng/μg
Shipping Condition Wet Ice
Expression System E. coli
Protein Family Cytokines & Receptors
Protein Form Recombinant, Ligand
Form Lyophilized
Protein Subtype GM-CSF, G-CSF, & M-CSF
Research Category Cardiovascular Research, Stem Cell Research, Differentiation, Inflammation
Product Line Gibco™
Purity or Quality Grade ≥98% by SDS-PAGE and HPLC
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WARNING: Cancer - www.P65Warnings.ca.gov
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