Invitrogen™ Mouse MIP-1 beta/CCL4 ELISA Kit

Description

ELISA

Mouse MIP-1 beta/CCL4 quantitates mouse MIP-1 beta/CCL4 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse MIP-1 beta/CCL4.

CCL4 (macrophage inflammatory protein 1-beta, MIP1B) belongs to the intercrine beta (chemokine CC) family. Functionally, CCL4 is involved in chemotactic and proinflammatory effects, and homeostasis. CCL4 is produced by macrophages upon stimulation by bacterial endotoxins. CCL4 recruits and stimulates various inflammatory cells at sites of inflammation. CCL4 is produced by lymphocytes, macrophages and dendritic cells. Both CCL4 and the related protein CCL3 participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells. While both CCL4 and CCL3 exert similar effects on monocytes, their effect on lymphocytes differ, with CCL4 selectively attracting CD4+ lymphocytes and CCL3 selectively attracting CD8+ lymphocytes. Additionally, both have been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. The processed form of CCL4 can induce down-modulation of surface expression of the chemokine receptor CCR5, thus inhibiting the CCR5-mediated entry of HIV-1 in T cells. CCL4 binds with high affinity to CCR5 receptors.

Specifications

• Accession Number: ACT2; ACT-2; AT744.1; CC chemokine ligand 4; C-C motif chemokine 4; C-C motif chemokine ligand 4; CCL4; chemokine (C-C motif) ligand 4; G-26; G-26 T-lymphocyte-secreted protein; HC21; immune activation gene 2; immune activation protein 2; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; macrophage inflammatory protein-1 beta; MGC104418; MGC126025; MGC126026; MIP-1 beta; Mip1b; MIP-1 B; Mip1-b; MIP1B1; MIP1beta; MIP-1 BETA; MIP-1-beta; MIP1-beta; MIP-1-beta(1-69); MIP-1-beta(3-69); PAT 744; Protein H400; putative MIP-1 beta protein; RP23-320E6.8; SCY4A; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4; small inducible cytokine A4 (homologous to mouse Mip-1 b); small-inducible cytokine A4; T-cell activation protein 2
• Assay Range: 4.5 to 1,000 pg/mL
• Assay Sensitivity: 4.5 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 20303
• Gene Symbol: CCL4
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CCL4
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins