Invitrogen™ Mouse Semaphorin 4D/CD100 ELISA Kit

Description

ELISA

Mouse Semaphorin 4D/CD100 quantitates mouse Semaphorin 4D/CD100 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse Semaphorin 4D/CD100.

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Specifications

• Accession Number: A8; BB18; C9orf164; CD100; coll-4; GR3; M-Sema G; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4 D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4 D; sema J; Sema4d; Semacl2; SEMAJ; semaphorin 4 D; semaphorin H; semaphorin-4 D; Semaphorin-C-like 2; semaphorin-J; Semcl2
• Assay Range: 23 to 5,000 pg/mL
• Assay Sensitivity: 23 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 20354
• Gene Symbol: Sema4d
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: CD100 (SEMA4D)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins