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Mouse Sonic Hedgehog/Shh C-Terminus Antibody, R&D Systems™
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Catalog No. AF445
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100 μg
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AF445 100 μg
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Catalog No. AF445 Supplier R&D Systems Supplier No. AF445
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Description

Goat Polyclonal Antibody has been used in 8 publications

Sonic Hedgehog/Shh Polyclonal specifically detects Sonic Hedgehog/Shh in Mouse samples. It is validated for Western Blot, Immunohistochemistry.

Specifications

Antigen Sonic Hedgehog/Shh
Applications Western Blot, Immunohistochemistry
Classification Polyclonal
Concentration LYOPH
Conjugate Unconjugated
Dilution Western Blot 1 ug/mL, Immunohistochemistry 5-15 ug/mL
Gene Accession No. Q62226
Gene Alias HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
Gene Symbols SHH
Host Species Goat
Immunogen E. coli-derived recombinant mouse Sonic Hedgehog Cys25-Ser437 with a Lys122Arg substitution Accession # Q62226
Purification Method Affinity Purified
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 6469
Test Specificity Detects mouse Shh C-terminal peptide in direct ELISAs and Western blots. In these formats, less than 5% cross-reactivity with recombinant mouse (rm) Dhh C-terminal peptide is observed and less than 1% cross-reactivity with a 6X histidine-tagged rmShh N-terminal peptide (amino acids 25-198) is observed. Detects human and mouse Sonic Hedgehog protein in Western blots. Shh C-terminal peptide specific IgG was purified by first passing the goat sera over a mouse Shh N-terminal peptide affinity column. The unbound fraction from the mouse Shh N-terminal peptide affinity column was subsequently purified using a mouse Shh C-terminal peptide affinity column.
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Target Species Mouse
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype IgG
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