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Mouse Sonic Hedgehog/Shh N-Terminus Antibody, R&D Systems™
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Catalog No. MAB4641500
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Catalog No. Quantity
MAB4641500 500 μg
MAB4641SP 25 μg
MAB4641100 100 μg
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Catalog No. MAB4641500 Supplier R&D Systems Supplier No. MAB4641500
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Description

Rat Monoclonal Antibody has been used in 2 publications

Sonic Hedgehog/Shh Monoclonal specifically detects Sonic Hedgehog/Shh in Mouse samples. It is validated for Western Blot, Immunohistochemistry, Neutralization, ELISA Capture (Matched Antibody Pair).

Specifications

Antigen Sonic Hedgehog/Shh
Applications Western Blot, Immunohistochemistry, Neutralization, ELISA
Classification Monoclonal
Clone 171018
Conjugate Unconjugated
Dilution Western Blot 2 ug/mL, Immunohistochemistry 8-25 ug/mL, Neutralization 5-15 ug/mL, ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. Q62226
Gene Alias HHG1, HHG-1, HLP3, HPE3, MCOPCB5, MCOPCB5sonic hedgehog (Drosophila) homolog, Shh, ShhNC, SMMCI, SMMCIsonic hedgehog homolog (Drosophila), sonic hedgehog, sonic hedgehog homolog, sonic hedgehog protein, TPT, TPTPS
Gene Symbols SHH
Host Species Rat
Immunogen E. coli-derived recombinant mouse Sonic Hedgehog Cys25-Gly198 (Lys122Arg) Accession # Q62226
Purification Method Protein A or G purified from hybridoma culture supernatant
Quantity 500 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 6469
Test Specificity Detects mouse Sonic Hedgehog in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Dhh (N-terminus; aa 23-198) is observed and no cross-reactivity with rmIhh (N-terminus; aa 66-240), rmDhh (C-terminus; aa 199-396), or rmShh (C-terminus; aa 199-437) is observed.
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Mouse
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Supernatant
Isotype IgG2a
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