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Description
Activity: ∽1.0U/mg. Unit Definition: One unit causes hydrolysis of one micromole of p-nitrophenyl phosphate per minute at pH 9.6 and 25°C. Freeze-dried powder.
Specifications
Specifications
| Concentration | ≥1 U/mg |
| Color | Off-White |
| pH | 9.8 |
| For Use With (Application) | Used to remove 5'-phosphate groups from Nucleic Acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulation |
| Content And Storage | -20°C |
| Source | Calf intestine |
| Quantity | 100 kU |
| Product Type | Alkaline Phosphatase |
| Molecular Weight (g/mol) | 140000 |
| Inhibitors | Acidification to 4.5 pH reversibly inactives the Enzyme, Inorganic Phosphate, chelating agents, EDTA |
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