MP Biomedicals™ Collagenase Type II from Clostridium histolyticum

Catalog No.	Quantity
ICN10050201	1 g

Catalog No. ICN10050201 Supplier MP Biomedicals™ Supplier No. 0210050201

Description

Collagenase used for collagen structural and biosynthetic studies

Collagenase degrades native helical collagen fibrils. It has an important role in connective tissue metabolism and is produced by specific cells involved in repairs and remodelling processes. It is a type II enzyme that contains greater clostripain activity.

• Specific activity:> 125 units/mg solid
• Unit definition: Liberates one μmole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5
• Presentation: brown shiny flakes
• Format: lyophilized powder
• pH: 7-9 for the A-a enzyme (optimum pH)
• Solubility: Soluble in water
• Pack size: 1g

Specifications

• Concentration: 1 mg/mL
• Color: Brown
• pH: 7 to 9
• For Use With (Application): Collagen structural and biosynthetic studies, release of cells from animal tissues
• Storage Buffer: 1mg/mL of Enzyme in 0.05M TES with 0.36mM Calcium Chloride, pH 7.5
• Content And Storage: 4°C
• Source: Clostridium histolyticum
• Quantity: 1 g
• Product Type: Collagenase Type II
• Molecular Weight (g/mol): 105000 and 57400
• Inhibitors: Metal chelating agents such as Cysteine, EDTA or o-Phenanthroline
• Form: Lyophilized Solid