MilliporeSigma™ MPK-3 (DUSP6), Mouse, Unlabeled, Clone: 5H6.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1726

Catalog No. MABS1726

Description

Dual specificity protein phosphatase 6 (EC 3.1.3.16; EC 3.1.3.48; UniProt Q16828; also known as Dual specificity protein phosphatase PYST1, MAP kinase phosphatase 3, Mitogen-activated protein kinase phosphatase 3, MKP-3) is encoded by the DUSP6 (also known as HH19, MKP3, PYST1) gene (Gene ID 1848) in human. MPK-3 (DUSP6) belongs to the family of dual-specificity phosphatases (DUSPs) that mediate dephosphorylation of MAPKs on threonine/serine and tyrosine residues. DUSP6 is upregulated in HER2+ carcinomas and is among several DUSPs thought to be involved in breast cancer metastasis. DUSP1, DUSP4, and DUSP6 are induced during epithelial-to-mesenchymal transition (EMT) and are involved in forming and maintaining breast cancer stem cells (CSCs), DUSP1 knockdown reduces CSC formation, while DUSP4 or DUSP6 knockdown enhances CSC formation. PKC CK2alpha is reported to phosphorylate DUSP6 and increase its dephosphatase activity. ERK1/2, on the other hand, can phosphorylate DUSP6 to promote its proteosomal degradation.

Specifications

• Antigen: MPK-3 (DUSP6)
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse IgG2bκ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: Q16828
• Immunogen: GST-tagged recombinant human MPK-3 (DUSP6) rhodanese domain fragment.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_001937
• Target Species: Human
• Form: Purified

• Applications: Western Blot
• Clone: 5H6.1
• Conjugate: Unconjugated
• Gene: DUSP6, HH19, MKP3, PYST1
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 5H6.1 targets an epitope within the rhodanese domain present in both spliced isoforms of human MPK-3 (DUSP6) reported by UniProt (Q16828).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG2b κ

For Research Use Only. Not for use in diagnostic procedures.