Invitrogen™ MRP2 Monoclonal Antibody (M2III-5)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA135678

Catalog No. 01-671-084

Description

The antibody reacts with an internal epitope of MRP2, a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. M2III-5 was raised against a fusion protein of the bacterial maltose binding protein and rat Mrp2, containing the 202-amino acid COOH terminal end of the transporter protein. The Mab detects rat, mouse and human MRP2. M2III-5 does not cross-react with the human MDR1 P-gp, MRP1, MRP3 orMRP5 gene products.

This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: MRP2
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: Tissue culture supernatant with 0.7% BSA and 0.1% sodium azide
• Gene Accession No.: Q63120, Q8VI47, Q92887
• Gene Symbols: ABCC2
• Immunogen: 202-amino acid COOH terminal of rat Mrp2.
• Regulatory Status: RUO
• Gene ID (Entrez): 1244, 12780, 25303
• Content And Storage: 4°C
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: M2III-5
• Conjugate: Unconjugated
• Gene: ABCC2
• Gene Alias: ABC30; Abcc2; AI173996; ATP binding cassette subfamily C member 2; ATP-binding cassette sub-family C member 2; ATP-binding cassette, subfamily C (CFTR/MRP), member 2; ATP-binding cassette, sub-family C (CFTR/MRP), member 2; calicular multispecific organic anion transporter; canalicular multidrug resistance protein; Canalicular multispecific organic anion transporter 1; Cmoat; CMOAT1; Cmrp; DJS; Human MRP2; KIAA1010; MRP2; multidrug resistance associated protein 2; multidrug resistance protein 2; multidrug resistance-associated protein 2
• Host Species: Mouse
• Quantity: 1 mL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b

Safety and Handling

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