CD81 Hamster anti-Mouse, Brilliant Violet 421, Clone: Eat2, BD Optibuild™

Hamster Monoclonal Antibody

Supplier:  BD Biosciences 740060

Catalog No. BDB740060

Description

The Eat2 monoclonal antibody specifically binds to CD81, a 26-kDa nonglycosylated member of the transmembrane 4 integral membrane protein superfamily, expressed by many types of cells. For example, CD81 participates with CD19 and CD21 in the signal transduction complex associated with the B-cell receptor on human B lymphocytes and with the CD4 and CD8 co-receptors on human thymocytes and T lymphocytes. In mouse fetal thymic organ culture, interactions of immature thymocytes with CD81 expressed by thymic stromal cells are required to induce development of T cells with αβ T-cell receptors. Furthermore, CD81 has been shown to play a role in the regulation of rat mastcell degranulation. Despite its important roles in the immune response and wide tissue distribution, CD81-deficient mice are born without obvious developmental abnormalities. However, these mice have abnormal immune responses, and impaired fertility. Eat2 mAb cross-reacts with the rat CD81 antigen.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

Specifications

• Antigen: CD81
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Description: TAPA-1; Tspan28; Tapa1; Tapa-1; Target of the antiproliferative antibody 1
• Host Species: Hamster
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG κ

• Applications: Flow Cytometry
• Clone: Eat2
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: CD81+ mouse B lymphoma 38C13
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only