MSH2 Rabbit anti-Human, Mouse, Polyclonal, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc A300452A

Catalog No. 50-155-7651

Description

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Germline mutations in human mismatch repair genes (hMSH2, hMSH6, hMLH1, hPMS2) account for majority of the hereditary non-polyposis colorectal carcinoma (HPNCC). CpG dinucleotides in the hMSH2 and hMLH1 genes are hotspots for HNPCC mutations. These mutations cause a mismatch repair deficiency resulting in a mutator phenotype where the replication errors are not repaired. Microsatellites/simple repeatative sequences are prone to this type of replication errors and instability of these microsatellites correlates with the occurance of HPNCC. hMSH2 binds to another MutS homolog protein GTBP to form a heterodimeric complex called hMutSbeta, which binds to insertion/deletion loops in DNA.

Specifications

• Antigen: MSH2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MSH2
• Gene Alias: COCA1, FCC1, HNPCC, HNPCC1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17685, 4436
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 1 mg/ml
• Formulation: phosphate, tris citrate with 0.09% sodium azide; pH 7-8
• Gene Accession No.: P43246, P43247
• Gene Symbols: MSH2
• Immunogen: Between 875 and C-term
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG