Invitrogen™ MST2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5121052

Catalog No. PIPA5121052

Description

Positive test controls include: 293T, HeLa, Jurkat, MCF7, NIH/3T3, Mouse liver, Mouse brain, Mouse kidney. The target is usually found in the following locations: Cytoplasm, Nucleus. Immunogen sequence: SHTMVKTSVE SVGTMRATST MSEGAQTMIE HNSTMLESDL GTMVINSEDE EEEDGTMKRN ATSPQVQRPS FMDYFDKQDF KNKSHENCNQ NMHEPFPMSK NVFPDNWKVP QDGDFDFLKN LSLEELQM.

STK3 (MST2) is a STE kinase family member that acts upstream of the MAP kinase signaling pathways, and is known to be activated by the proapoptotic stimuli staurosporine and FAS ligand. STK3 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation.

Specifications

• Antigen: MST2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: STK3
• Gene Alias: 0610042I06Rik; KB-1458E12.1; KRS1; Mammalian STE20-like protein kinase 2; mess1; MST; Mst2; MST-2; MST2/C; MST2/N; Mst3; protein kinase homolog; serine/threonine kinase 3; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); serine/threonine-protein kinase 3; Serine/threonine-protein kinase 3 20kDa subunit; Serine/threonine-protein kinase 3 36kDa subunit; Serine/threonine-protein kinase Krs-1; Ste20; STE20-like kinase MST2; STK3; wu:fc19e11; zgc:55383
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 56274, 65189, 6788
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot, Immunocytochemistry
• Concentration: 1.05 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: O54748, Q13188, Q9JI10
• Gene Symbols: STK3
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 323-450 of human STK3/MST2 (NP_006272.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG