MT-ND2, Mouse anti-Human, Clone: 9E12-1B3, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABS204725UG

Catalog No. MABS204725

Description

Anti-MT-ND2, clone 9E12-1B3, Cat. No. MABS2047, is a mouse monoclonal antibody that detects NADH-ubiquinone oxidoreductase chain 2 and has been tested for use in Western Blotting.

NADH-ubiquinone oxidoreductase chain 2 (UniProt: P03891; also known as EC: 1.6.5.3, NADH dehydrogenase subunit 2, MT-ND2) is encoded by the MT-ND2 (also known as MTND2, NADH2, ND2) gene (Gene ID: 4536) in human. MT-ND2 is a mitochondrial inner membrane protein that forms the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). It is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (Coenzyme Q10) that participates in the generation of a proton gradient, which is then used for ATP synthesis. Defects in MT-ND2 gene are known to cause of Leber hereditary optic neuropathy (LHON) that is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.

Specifications

• Antigen: MT-ND2
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.
• Gene Symbols: MT-ND2;MTND2;NADH2;ND2
• Immunogen: Synthetic peptide corresponding to the N-terminus of human MT-ND2.
• Quantity: 25 μg
• Research Discipline: Signaling
• Test Specificity: Clone 9E12-1B3 detects NADH-ubiquinone oxidoreductase chain 2 (MT-ND2) in mitochondria from human cells. It targets an epitope within the N-terminal region.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG2a κ

• Applications: Western Blot
• Clone: 9E12-1B3
• Dilution: Western Blotting Analysis: 2μg/mL from a representative lot detected MT-ND2 in mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR USA).
• Gene Alias: NADH-ubiquinone oxidoreductase chain 2;EC: 1.6.5.3;NADH dehydrogenase subunit 2;MT-ND2
• Host Species: Mouse
• Purification Method: Protein L
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Form: Purified