MT-ND4, Mouse anti-Human, Clone: 9E4-2D8, Millipore Sigma™

Catalog No.	Quantity
MABS199425	25 μg

Catalog No. MABS199425 Supplier MilliporeSigma Supplier No. MABS199425UG

Description

Mouse Monoclonal Antibody

Anti-MT-ND4, clone 9E4-2D8, Cat. No. MABS1994,is a mouse monoclonal antibody that detects NADH-ubiquinone oxidoreductase chain 4 and has been tested for use in Western Blotting.

NADH-ubiquinone oxidoreductase chain 4 (UniProt: P03905; also known as EC: 1.6.5.3, NADH dehydrogenase subunit 4, MT-ND4) is encoded by the MT-ND4 (also known as MTND4, NADH4, ND4) gene (Gene ID: 4538) in human. MT-ND4 is an inner mitochondrial membrane protein that belongs to the complex I subunit 4 family. It is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). The complex couples the oxidation of NADH and the reduction of ubiquinone (Coenzyme Q10), to the generation of a proton gradient, which is then used for ATP synthesis. The complex can be dissociated into two main sub-complexes, corresponding to the "ankle" of the boot, and the "foot" of the boot. The ankle is thought to protrude from the membrane so as to be predominantly in the aqueous phase on the matrix side. It contains the binding site for NAD(H), and the input electron transfer chain. The foot (hydrophobic) is membrane bound and contains a catalytic site at which ubiquinone is reduced. Defects in MT-ND4 gene are known to cause Leber hereditary optic neuropathy (LHON) that is characterized by acute or subacute loss of central vision due to optic nerve dysfunction. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes. Other diseases associated with MT-ND4 gene mutations are age-related macular degeneration, mesial temporal lobe epilepsy, and cystic fibrosis.

Specifications

• Antigen: MT-ND4
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 9E4-2D8
• Conjugate: Unconjugated
• Dilution: Western Blotting Analysis: 4μg/mL from a representative lot detected MT-ND4 in mitochondria from Human Neonatal Dermal Fibroblasts and mitochondria from Human neonatal dermal fibroblasts depleted of mtDNA. (Courtesy of  Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR, USA ).
• Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.
• Gene Alias: NADH-ubiquinone oxidoreductase chain 4;EC: 1.6.5.3;NADH dehydrogenase subunit 4
• Gene Symbols: MT-ND4;MTND4;NADH4;ND4
• Host Species: Mouse
• Immunogen: KLH-conjugated linear peptide from the C-terminal region.
• Purification Method: Protein L
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Test Specificity: Clone 9E4-2D8 detects NADH-ubiquinone oxidoreductase chain 4 in mitochodria isolated from human cells.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2a