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Invitrogen™ mTOR Polyclonal Antibody
Rabbit Polyclonal Antibody
Supplier: Invitrogen™ PA1188
Description
Western blot analysis of PA1-188 detects mTOR at ~250 kDa in human and non-human primate cells, slightly below the theoretical MW of ~280kDa. Using a Tris-Acetate SDS gel for high molecular weight protein separation, and a nitrocellulose membrane for protein transfer are necessary for optimal WB results. Immunofluorescence analysis shows cytoplasmic and some nuclear staining of mTOR in human B3 and U-2 OS cells.
FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.
Specifications
mTOR | |
Polyclonal | |
Unconjugated | |
MTOR | |
2610315D21Rik; AI327068; angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; flat; FRAP; FRAP1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mechanistic target of rapamycin kinase; Mtor; m-TOR; mTORC1; Raft1; Rapamycin and FKBP12 target 1; rapamycin and FKBP12 target-1 protein; rapamycin associated protein FRAP2; rapamycin target protein 1; RAPT1; serine/threonine-protein kinase mTOR; SKS | |
Rabbit | |
Protein G | |
RUO | |
2475 | |
-20°C | |
Liquid |
Western Blot, Immunocytochemistry | |
1 mg/mL | |
PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide | |
P42345 | |
MTOR | |
Recombinant protein corresponding to amino acids 2447-2549 of human mTOR expressed in E. coli. | |
100 μg | |
Primary | |
Human, Monkey | |
Antibody | |
IgG |
Safety and Handling
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